Protein_Domain

Part:BBa_J70554:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-02-17)


Split GFP C-terminal domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 176


Design Notes

added an ATG at the start of the sequence


Source

Pcr'd from J04430

Forward primer (J70554-f): ttcgaattcgcggccgcttctagATGaagaatggaatcaaagttaacttcaaaattag

Reverse primer (J70554-r): gtttcttcctgcagcggccgctactagtataaacgcagaaaggcccacccgaa

References